【英文标准名称】:StandardPracticeforTissueCryosectionAnalysiswithSIMS
【原文标准名称】:用次级离子质谱法(SIMS)进行组织低温截面分析的标准实施规程
【标准号】:ASTME1880-2006
【标准状态】:现行
【国别】:
【发布日期】:2006
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E42.06
【标准类型】:(Practice)
【标准水平】:()
【中文主题词】:生物工艺学;低温学;成象;质谱法;微生物分析;光学;组织
【英文主题词】:SIMS
【摘要】:PressingcryosectionsflatontoaconductingsubstratehasbeenoneofthemostchallengingproblemsinSIMSanalysisofcryogenicallypreparedtissuespecimens.Frozencryosectionsoftencurlorpeeloff,orboth,fromthesubstrateduringfreeze-drying.ThecurlingofcryosectionsresultsinanunevensamplesurfaceforSIMSanalysis.Furthermore,iffreeze-driedcryosectionsarenotattachedtightlytothesubstrate,theimpactoftheprimaryionbeammayresultinfurthercurlingandevendislodgingofthecryosectionfromthesubstrate.TheseproblemsrenderSIMSanalysisdifficult,frustratingandtimeconsuming.Theuseofindiumasasubstrateforpressingcryosectionsflathasprovidedapracticalapproachforanalyzingcryogenicallypreparedtissuespecimens.(1)TheproceduredescribedhereinhasbeensuccessfullyusedforSIMSimagingofcalciumandmagnesiumtransportandlocalizationofanticancerdrugsinanimalmodels(2,3,4,5)Theproceduredescribedhereisamenabletosofttissuesofbothanimalandplantorigin.1.1ThispracticeprovidestheSecondaryIonMassSpectrometry(SIMS)analystwithamethodforanalyzingtissuecryosectionsintheimagingmodeoftheinstrument.Thispracticeissuitableforfrozen-freeze-driedandfrozen-hydratedcryosectionanalysis.1.2Thispracticedoesnotdescribemethodsforoptimalfreezingofthespecimenforimmobilizingdiffusiblechemicalspeciesintheirnativeintracellularsites.1.3Thispracticedoesnotdescribemethodsforobtainingcryosectionsfromafrozenspecimen.1.4Thispracticeisnotsuitableforanyplasticembeddedtissues.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:A40
【国际标准分类号】:17_180_01
【页数】:2P.;A4
【正文语种】: